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Surf. Sci. Spectra 8, 163 (2001); http://dx.doi.org/10.1116/11.20020301 (22 pages)

Analysis of Poly(amino acids) by Static Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

Newton T. Samuel, M. S. Wagner, K. D. Dornfeld, and David G. Castner

Department of Chemical Engineering and Bioengineering, University of Washington, Box 351750, Seattle, WA 98195

This study presents the static time-of-flight secondary ion mass spectrometry (TOF-SIMS) spectra of 15 poly(amino acids), solvent or spin cast onto either mica or silicon substrates. These poly(amino acid) spectra are useful for interpreting the complex static TOF-SIMS spectra obtained from adsorbed protein films and peptide-functionalized surfaces. Previous studies have reported poly(amino acid) spectra acquired with a quadrupole SIMS instrument. The spectra obtained with a TOF-SIMS instrument in this study have significantly higher sensitivity and mass resolution, which are essential for producing good, high-quality reference spectra. © 2001 American Vacuum Society.

© 2001 American Vacuum Society

KEYWORDS and PACS

PACS

  • 82.80.Ms

    Mass spectrometry (including SIMS, multiphoton ionization and resonance ionization mass spectrometry, MALDI)

  • 87.14.E-

    Proteins

  • 82.35.Pq

    Biopolymers, biopolymerization

PUBLICATION DATA

ISSN

1055-5269 (print)  
1520-8575 (online)

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